growth hormone releasing factor meaning in English
促生长素释放因子
生长激素释放因子激素
Examples
- Extro growth hormone releasing factor mediated by protoplast could more than 28 days retention in rabbit and mouse muscle that is condition of long - time expression of grf
通过提取注射部位dna后进行pcr鉴定,可以确认原生质体介导的grf基因至少可以在受试动物体内滞留28天以上,为grf的长期表达提供了条件。 - The result of investigation showing : extro growth hormone releasing factor mediated by protoplast expressed in rabbit muscle and mouse muscle , products of expression were detected by pcr rt - pcr and elisa
研究结果表明,以原生质体介导的外源性grf基因可以在家兔和小鼠肌肉中表达,通过pcr 、 rt - pcr和elisa都可以检测到表达产物。 - Growth hormone releasing factor is polypeptide hormone releasing by animal hypothaiami . it can induction synthesis and excretion of growth hormone which rise growth hormone level of animal . we large - scale cultured jm - 109 strain recombinant plasmid of grf
生长激素释放因子( growthhormonereleasingfactor , grf )是由动物下丘脑合成并分泌的一种多肽激素,能特异性的诱导生长激素的合成与分泌,提高体内生长激素的水平。 - Then the plasmid was extracted from them and determined by dna calculator . the protoplast that contain growth hormone releasing factor injected into rabbit muscle and mouse muscle after it were treated by 1 % glutaral , pay it to electric stimulation in muscle of injection site and extract omni - rna from injection site of rabbit muscle , expression of grf were detected by reverse transcription and pcr ; ratio of expression of grf were detected by elisa . extract dna form injection site of mouse muscle to research time of expression
本实验是将含grf重组质粒的jm109菌株大量培养,用碱裂变法提取质粒,用dnacaculator定量;制备含grf的原生质体,经1的戊二醛处理后注射于家兔肌肉,在注射部位给予电刺激,提取总rna ,用rt - pcr检测grf在肌肉中的表达;用elisa法定性检测grf在肌肉中蛋白质水平的表达;将该原生质体注射于小鼠肌肉中,定期提取dna ,初步探讨原生质介导的外源性grf在小鼠肌肉中的表达时间。
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